Coding

Part:BBa_K163000:Design

Designed by: Nora Yucel   Group: iGEM08_University_of_Chicago   (2008-08-22)


T4 phage gene T for self-lysis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A protein that could effectively trigger cell-lysis in E. coli upon selective induction. When looking for a cell-lysis pathway, system had to be shown to work in E. coli, be non-lethal until induction, and act quickly once triggered. This particular gene was designed by Morita, et al to turn on following inducible expression of a target protein, a feature that could be used to easily purify a protein of interest. Furthermore, Morita, et al report that cell-lysis proceeded efficiently at both mid or late logarithmic growth phase.


Source

Sequence from paper "Programmed Escherichia coli Cell Lysis by Expression of Cloned T4 Phage Lysis Genes " by Morita, et al. Biotechnol. Prog., 17 (3), 573 -576, 2001. Gene bank accession number Y00408.

References